Product Description

The HA TECH SARS-CoV-2 RT-PCR Multiplex Diagnostic Kit is a reliable real-time RT-PCR test designed for the qualitative detection of SARS-CoV-2 RNA in upper respiratory specimens, including nasopharyngeal and oropharyngeal swabs. The kit targets WHO-recommended genes (N gene, E gene, and ORF1ab gene) and includes an internal control (RNaseP gene) to ensure sample quality. It is supplied with a positive control (purified SARS-CoV-2 RNA) for accuracy validation and a no template control to verify procedural and reagent integrity. Designed for use by trained clinical laboratory professionals skilled in real-time PCR and in vitro diagnostic techniques, this kit provides a comprehensive and efficient solution for reliable SARS-CoV-2 diagnostics.

Advantages

  • Rapid results delivered in approximately 45 minutes post-extraction
  • Ultra-sensitive detection with a limit of 0.8–4 copies per µL, ensuring reliable results even at low target concentrations
  • High specificity with no cross-reactivity to SARS-CoV-1 or common respiratory pathogens
  • Broad compatibility with most multichannel Real-Time PCR Thermal Cyclers and RNA extraction methods for seamless integration into your workflow

Components Tube Vol (μl) Reagent Vol (μl)
NAR-01 PCR Master Mix 1500 1000
NAR-02 PCR Enzyme Mix 500 100
NAR-03 PCR Multiplex Mix 500 400
NAR-04 PCR Positive Control 500 50
NAR-05 PCR Negative Control 1500 1000
Target gene Fluorophore (λemm)
N gene FAM (520 nm)
E gene HEX (556 nm)
ORF1ab gene ROX (602 nm)
Internal control – RNaseP Cy5 (670 nm)

Shipping temperature 2~8°C.

  • All reagents should be stored at -20±5°C with protection from light. Avoid multiple freeze-thaw cycles. The reagents are stable for 6 months when stored at the recommended condition.
  • Expiration date is 12 months after production date.
  • The expiration date will not change if the kit is opened and stored at the recommended conditions.
  • The expiration date will not change if the kit is transported with ice-packs for 4 days and/or treated with 10 freeze-thaw cycles.
Product Cat. No.
SARS-CoV-2 RT PCR Multiplex Diagnostic Kit (100 Reactions) NAT-01

Analytes: N gene, E gene, Orf1ab, RnaseP gene

Specimens: Nasopharyngeal and Oropharyngeal swabs

Contact us for detailed IFU:  info@ha-tech-ltd.com

Download: Safety Data Sheet