The HA TECH SARS-CoV-2 RT-PCR Multiplex Diagnostic Kit is a real time RT-PCR test intended for the qualitative detection of RNA from the SARS-CoV-2 virus in upper respiratory specimens such as nasopharyngeal swabs and oropharyngeal swabs. The real-time RT-PCR test is intended for use by qualified clinical laboratory personnel specifically trained in the techniques of real-time PCR and in vitro diagnostic procedures.
This product uses well characterised primers and probes for the N gene, E gene and Orf1ab and combines them into an optimised multiplex that reduces reagent use, sample volume needed and improves detection as samples may be picked up that were otherwise missed with a single gene target. Our primers and probes have also been aligned with sequences of the new UK B1.1.7, South African B1.351, UK/South Africa B1.525, California B1.429/B1.427, Brazil B1.1.28 and India B1.617 variants. There were no mismatches which suggests our assays will not miss the new variants. RNaseP internal control (RNaseP gene) provides a nucleic acid extraction procedural control and a secondary negative control. Positive control (purified SARS-CoV-2 genomic RNA) provides a reverse transcription and PCR control to validate the post extraction procedure and reagent integrity.
|Components||Tube Vol (μl)||Reagent Vol (μl)|
|NAR-01 PCR Master Mix||1500||1000|
|NAR-02 PCR Enzyme Mix||500||100|
|NAR-03 PCR Multiplex Mix||500||400|
|NAR-04 PCR Positive Control||500||50|
|NAR-05 PCR Negative Control||1500||1000|
Target Gene Fluorophores
|Target gene||Fluorophore (λemm)|
|N gene||FAM (520 nm)|
|E gene||HEX (556 nm)|
|ORF1ab gene||ROX (602 nm)|
|Internal control – RNaseP||Cy5 (670 nm)|
Storage And Shelf Life
Shipping temperature 2~8°C.
- All reagents should be stored at -20±5°C with protection from light. Avoid multiple freeze-thaw cycles. The reagents are stable for 6 months when stored at the recommended condition.
- Expiration date is 12 months after production date.
- The expiration date will not change if the kit is opened and stored at the recommended conditions.
- The expiration date will not change if the kit is transported with ice-packs for 4 days and/or treated with 10 freeze-thaw cycles.
|SARS-CoV-2 RT PCR Multiplex Diagnostic Kit (100 Reactions)||NAT-01|
Analytes: N gene, E gene, Orf1ab, RnaseP gene
Specimens: Nasopharyngeal and Oropharyngeal swabs